SINγ-RV-TGM1 rescues TG1 expression and activity in the ΔTGM1 human cellular model
(A) Representative images of TG1 immunofluorescence analysis of wild-type, ΔTGM1, and SINγ-RV-TGM1-transduced keratinocytes after 7-days cultivation. DAPI (blue) stains nuclei. Scale bars, 50 μm. (B) Optical micrographs of the isolated cornified cell envelopes in wild-type and SINγ-RV-TGM1-transduced keratinocytes. Note that typical envelopes were not detected in ΔTGM1 keratinocytes, and only a few cell fragments were observed. Scale bar, 20 μm. (C) Immunostaining detection of TG1 protein (top, green) and TG1 in situ enzymatic activity (bottom, red) in cryosections of collagen-based 3D SEs from wild-type, ΔTGM1, or SINγ-RV-TGM1-transduced keratinocytes. DAPI (blue) stains nuclei. A white dotted line marks the epidermal-dermal junction. Scale bars, 50 μm (n = 3, pictures are representative of what was observed in at least three independent samples or replicates).