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Determination of SE301 binding affinity for RXFP1. (a,b) Flow cytometry binding data for SE301 (a) and Fc N297Q (b) using human RXFP1 and empty vector-transfected Expi293F cells. The KD for SE301 at human RXFP1 was calculated to be 122 ± 36 nM. Data are the mean ± s.e.m. from technical duplicates.
