Figure 3.
Characterization of SE301 purity, monodispersity, and stability. (a) Coomassie-stained SDS-PAGE gel of SE301 and Fc N297Q. (b) CRE-SEAP Gs signaling testing the activity of SE301 at human RXFP1 after incubations at RT for 0, 7, 14, 21, and 28 days, showing that SE301 retains activity after 4 weeks at RT. Data are normalized to the native relaxin-2 response at human RXFP1 and are the mean ± s.e.m. from technical triplicates. (c) Size exclusion profile for SE301 shows a monodisperse peak. (d) Differential scanning fluorimetry for SE301 determined its melting temperature to be 57 °C.