Fig. 2.

BRCC36 overexpression alleviates vascular calcification in mice. A The transfection efficiency of BRCC36 overexpression was verified by western blotting. n = 6 per group. B RUNX2, α-SMA and GAPDH immunoblotting in the vessel lysates of control and CKD of AAV-NC and AAV-BRCC36 OE mice, n = 6 per group. C Representative Von Kossa staining, Alizarin Red S staining, and IHC staining of RUNX2 images in aortas among all groups. Scale bar, 200 μm. n = 6 per group. D Representative IF images of RUNX2 and α-SMA in aortas among all groups. Scale bar, 100 μm. E RUNX2, α-SMA and GAPDH immunoblotting in the vessels lysates of control and CKD of AAV-NC and AAV-shBRCC36 mice, n = 6 per group. F Representative Von Kossa staining, Alizarin Red S staining, and IHC staining of RUNX2 antibody images in aortas among all groups. Scale bar, 200 μm. n = 6 per group. G Statistical analysis of RUNX2 was performed. H Representative IF images of RUNX2 and α-SMA in aortas among all groups. Scale bar, 100 μm. I Representative Alizarin Red S staining images of the whole aortas. Scale bar, 5 mm. Statistical significance was assessed using two tailed t-test (A) and 1-way ANOVA followed by Dunnett’s test (B, C, E, G). All values are presented as mean ± SD. *P < 0.05