Fig. 3.

BRCC36 regulates the osteogenic transdifferentiation of VSMCs in vitro. A Western blot analysis of RUNX2, BMP2, and α-SMA expression in VSMCs. n = 3 per group. B Photomicrographs of Alizarin Red S staining in VSMCs pretransfected with indicated treatment. C Calcium content in VSMCs from all of the experimental cohorts. n = 4 per group. D, E Western blot analysis of BMP2 and α-SMA expression in VSMCs. n = 4 per group. F Calcium content in VSMCs from all of the experimental cohorts (n = 4 per group). G Photomicrographs of Alizarin Red S staining in VSMCs pretransfected with indicated treatment. H Western blot analysis of RUNX2 and α-SMA expression in VSMCs. n = 4 per group. I Calcium content in VSMCs from all of the experimental cohorts. n = 4 per group. J Photomicrographs of Alizarin Red S staining in VSMCs pretreated with indicated treatment. K Quantitative real-time PCR analysis of OCN, ALP, and SM22α expression in VSMCs, which were pretreated with G5. Statistical significance was assessed using two tailed t-test (E) and 1-way ANOVA followed by Dunnett’s test (A, C, F, H, I, K). All values are presented as mean ± SD. *P < 0.05