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. 2024 Jul 16;39(9):2053–2066. doi: 10.1093/humrep/deae154

Figure 2.

Figure 2.

Mitochondrial metabolism in mouse granulosa cells (GCs) and cumulus–oocyte complexes (COCs) in response to ovulatory hormones. (A) Schematic representation of mitochondrial metabolism and inhibitory actions of rotenone (rote), antimycin A (anti), oligomycin (oligo), and Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP). (B) Oxygen consumption rate (OCR) (pmol/min) was measured in GCs (150K cells/well) collected from mice at 24 and 48 h after PMSG (P), and at 4, 8, and 12 h after hCG. Basal respiration (C); ATP production inferred from OCR (D); maximal respiration (E); spare respiratory capacity (F), protein leak (G), and coupling efficiency (H) were determined. N = 4–8 pools of cells from multiple mice collected at each timepoint. (I–O) OCR was measured in mouse COCs in parallel to the GCs. N = 3–13 pools of COCs from multiple mice collected at each timepoint. Data shown as mean ± SEM. Statistical analysis by one-way ANOVA with comparison to cells collected at PMSG 48 h. **P < 0.01; ***P < 0.001; ****P < 0.0001 compared to PMSG 48 h.