Figure 1.

Spheroid model (A) Representative images of GSC4, LN-18, and LN-229 spheroids cultured in the absence of a drug for 7 days. Viable proliferative cells of the outer layer are shown in green, hypoxicquiescence cells from the mantle are shown in turquoise and necrotic cells from the core are shown in blue. Temozolomide (TMZ) response by (B) spheroid size and (C) percent viable cells relative to total cell counts normalized to day 0 by Z-projection in the presence of TMZ or vehicle (DMSO) (n = 6, ****P ≤ .0001). (D) ICY BioImage Analysis Tool utilizing spot detection plugin. This detection method of Z-Projection (upper left) involves determining regions of interest (upper right), detection of viable cells (lower left) and detection of dead cells (lower right).