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. Author manuscript; available in PMC: 2024 Sep 4.
Published in final edited form as: Biochem J. 2020 Dec 11;477(23):4473–4489. doi: 10.1042/BCJ20200455

Figure 2. MtrA is methylated in Mtb.

Figure 2.

(a) Pictorial representation of MtrA domain organization showing the location of different methylated sites. (b) His6-MtrA was overexpressed and purified from Mtb. Purified protein was probed with anti-methyllysine antibody (upper panel) and anti-MtrA (lower panel) antibody. Histone was used as a positive control. (c) Structural representation of MtrA (PDB ID: 2GWR). Response regulator domain (blue), linker region (green), and DNA binding domain (pink) are visible with the three crucial methylated sites marked red.