Fig.3. ANP ameliorated eNOS phosphorylation in MGO-treated HUVEC.
Incubation with MGO attenuated eNOSSer1177 and Akt phosphorylation and promoted eNOSThr495 phosphorylation in HUVEC. Therefore, the eNOSSer1177/eNOSThr495 phosphorylation ratio decreased by MGO treatment (n=7 per group). However, ANP tended to increase eNOSSer1177 phosphorylation and significantly decrease the eNOSThr495 phosphorylation induced by MGO. Therefore, the eNOSSer1177/ eNOSThr495 phosphorylation ratio was significantly recovered in the presence of ANP (n=7 per group). ＊; P＜0.05, ＊＊; P＜0.01, and ＊＊＊; P＜0.001 vs. MGO-treated group. ANP, ANP-treated group; MGO, MGO-treated group; A+M, combination treatment group with ANP and MGO. All values are presented as the mean±SEM.