Fig.4. ANP ameliorated endothelium-dependent vascular relaxation impaired by MGO.

(A) MGO significantly impaired the endothelium-dependent vascular relaxation in the aortic segments obtained from C57BL/6 mice. Incubation with ANP ameliorated MGO-induced endothelial dysfunction. However, in the presence of L-NAME, the effect of ANP was abrogated (n=7, per group). (B) Both ANP and MGO did not affect the vascular response to SNP (n=7, per group). ^＊; P＜0.05, ^＊＊; P＜0.01, and ^＊＊＊; P＜0.001 vs. MGO group. NT: non-treated group, ANP: ANP-treated group, MGO: MGO-treated group, ANP＋MGO: combination treatment of ANP and MGO, ANP＋MGO＋L-NAME: combination treatment of ANP, MGO, and L-NAME. All values are presented as the mean±SEM.