Extended Data Fig. 10. Irf3-deficient hearts have greater fibroblast activation and protective matricellular responses.
(a) Nuclei isolation from WT and Irf3-deficient hearts were processed for snRNA-seq. Nuclei data sets were integrated to enable direct comparison of cell types. (b) Averaged heatmap identifying fibroblast subpopulations were further stratified into activated and not activated based on variable expression level of Postn. (c) UMAP of clustered fibroblasts from snRNA-seq integrated data of WT and Irf3−/− nuclei from D3 infarcted hearts in the left panel and separation of activated vs. non-activated fibroblast populations in the right panel (Wilcoxon-signed rank test, Bonferroni-adjusted P < 0.01). (d) Percent of activated fibroblasts out of total captured fibroblasts (n = 3 mice per condition). (e) Log normalized expression of fibroblast activation markers between those captured from Irf3-deficient and WT D3 MI hearts. Data were analysed using two-tailed unpaired t test (d, e). All data represented as mean ± s.e.m. *P > 0.05, **P > 0.005, ***P > 0.0005, ****P > 0.00005.