Extended Data Table 2.

Displacement binding parameters in κLight, δLight, and μLight cells

Displacement binding assays were carried out with membranes (100 μg) from μLight, δLight or κLight cells, [3H] diprenorphine (3 nM final concentration) and different ligands (0- 10 μM final concentration) as described in Methods. Data are Mean ± SE of 3 experiments in triplicate. Binding in the absence of cold ligand was taken as 100% bound. Binding in the presence of 10 μM ligand was taken as non-specific binding. % Specific binding at 10 μM = Total binding – non-specific binding. n.t.= not tested. * Displacement binding assays were carried out with CHO-MOR membranes (100 μg).