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. 2024 Sep 4;15:7731. doi: 10.1038/s41467-024-51577-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Comparison of detection of Domain 1 variants across different sample preparations (reported as a percentage of samples). Additional organ types are presented in the supplementary information. Kruskal–Wallis rank sum test was used for statistical analysis. B Comparison of percentage cancer cell content to variant allele frequency for selected actionable mutations. The solid black line represents the linear regression fit, and the shaded area around the line indicates the 95% confidence interval of the fit. A vertical red dotted line is demonstrated at variant allele frequency 0.1 to demonstrate that a significant number of mutations would be discarded if conventional bioinformatic filtering was applied. Top left panel plots EGFR variants associated with gefitinib sensitivity in lung cancer. Top right panel plots the KRAS G12C variant associated with gefitinib resistance in lung cancer. Bottom right panel plots PIK3CA variants in breast cancer and the bottom left panel plots BRAF V600E variant in all cancer groups included in the study. Correlation was assessed using Spearman’s Rank Correlation (two-sided test). FF fresh frozen, FF (PCR) fresh frozen with polymerase chain reaction, FFPE formalin fixed paraffin embedded.