Fig. 1.

Experimental design (A) Sample collection; venous and arterial blood collection in glass tubes (red-capped) and plastic-coated (white capped) collection tubes. (B) Preparation of L-PRF and liquid L-PRF glue. (C) In vitro set-up, culture (1 week and 2 weeks) of L-PRF membranes, preparation for histology and RT-qPCR, collection of medium for protein quantification of growth factors and D-dimers (ELISA), processing to single cell suspension of fresh and cultured membranes for single-cell week RNA sequencing and flow cytometry. (D) In vitro set up, culture and co- culture of liquid L-PRF glue with L-PRF membranes, processing to single cell suspension for flow cytometry