Fig. 2.

a Transcript level fold change of candidate genes (overexpressed stable cell pools/parental cells), normalized to housekeeping gene GAPDH. The data represent the mean and standard deviation of triplicate qPCR wells. b Primer design strategy for 5′/3′ junction PCR. c Verification of targeted integration at the AAVS1 locus by 5′/3′ junction PCR. d Gel electrophoresis image, sample name, and corresponding expected band size. HA-L represents left homology arm. HA-R represents right homology arm. Puro represents puromycin resistance gene sequence. CMV represents cytomegalovirus promoter. N/A represents no specific defined band size