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. 2024 Sep 4;15:7376. doi: 10.1038/s41467-024-51125-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Experimental procedure. b Distributions of tumor-derived rat glioma cells in fluorescence intensity (black histogram), fluorescence lifetime (blue histogram), and fluorescence lifetime gradient from nuclear periphery to center (red histogram). SP: subpopulation. n = 5732. c Comparison of subpopulations in morphological features of cells and their nuclei. TP: total population. Values between two samples represent effect sizes (Cohen’s d), with their standard errors <0.04. The violin plots display the median values with white dots, the first and third quartiles with box edges, and 1.5 times the IQR with whiskers. For the violin plots, the sample sizes are as follows: TP: n = 5732; SP1: n = 1054; SP2: n = 1991; SP3: n = 2687. The bar plot illustrates the percentage of cells with multiple nuclei calculated for each cell population, accompanied by the upper and lower bounds of its 95% confidence interval. Source data are provided as a Source Data file.