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. 2024 Sep 4;15(9):647. doi: 10.1038/s41419-024-07037-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Extracellular acidification rate (ECAR) was measured in MDA-MB-468 silenced with control oligos or siRNAs for TBC1D genes ECAR data were normalized on the number of cells. 2-DG: 2-Deoxy-D-glucose. The shown profiles (left) are representative of 3 independent experiments. Values are the mean ± SD n = 6. The bar graphs (right) report the parameters glycolysis and glycolytic capacity in cells silenced as indicated (data represent mean ± SD of 3 independent experiments, n = 18). B O₂ consumption rate (OCR) was measured in MDA-MB-468 silenced with control oligos or siRNAs for TBC1D genes. The shown profiles (left) are representative of 3 independent experiments. OCR data were normalized on the number of cells. FCCP: carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone, Rot/Ant: rotenone + antimycin. Values are the mean ± SD n = 6. The bar graphs (right) report the parameters basal respiration and maximal respiration in the cells silenced as on bottom (data represent mean ± SD of 3 independent experiments, n = 18). In A, B, *P < 0.05; **P < 0.01; ***P < 0.001; ns not-significant. C Energy map. Relative baseline ECAR and OCR data were plotted simultaneously to reveal overall relative metabolic profiles of MDA-MB-468 cells, variously silenced as indicated in the legend on the right. Data represent mean ± sem of 3 independent experiments, n = 18.