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. 2024 Aug 21;15:1444007. doi: 10.3389/fimmu.2024.1444007

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Copyright © 2024 Rayford, Gärtner, Ramnefjell, Lorens, Micklem, Aanerud and Engelsen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identity and composition of cell-types in NSCLC tumor samples. (A) Heatmap of mean phenotypic marker expression (min-max-scaled) in each of the 14 annotated cell-types detected by IMC. (B, C) Dotplots showing total abundance of each cell-type relative to all cells (whole-tissue) (B) and proportion of each immune cell-type relative to only immune cells in whole-tissue (C). (D, E) Single-cell segmented IMC images colored by cell-type in a non-squamous, ICI OS-low patient with high tAXL expression (Hscore=170) (D) and in a squamous, ICI OS-high, AXL Hscore-negative patient (E). Scale bars in μm.