Table 2.
Statistics on specific sample group comparisons
| Stimulation | Cutoff CD107a+ (%) | Sensitivity | Specificity | Youden | DP | Accuracy |
|---|---|---|---|---|---|---|
| EXO vs IEI | ||||||
| K562 | 5.3 | 93.1 | 87.0 | 0.81 | 1.08 | 89.3 |
| Anti-CD16 | 12.5 | 96.6 | 92.4 | 0.89 | 1.40 | 98.7 |
| Anti-CD3 | 23.5 | 94.8 | 92.4 | 0.88 | 1.29 | 93.3 |
| EXO vs HYPINF | ||||||
| K562 | 8.0 | 81.0 | 95.7 | 0.78 | 1.09 | 89.7 |
| Anti-CD16 | 14.3 | 98.4 | 94.6 | 0.93 | 1.67 | 96.1 |
| Anti-CD3 | 21.2 | 98.4 | 91.3 | 0.90 | 1.55 | 94.2 |
| EXO vs HD-CTRL | ||||||
| K562 | 9.9 | 97.8 | 98.0 | 0.96 | 1.84 | 97.9 |
| Anti-CD16 | 26.3 | 98.9 | 99.0 | 0.98 | 2.18 | 99.0 |
| Anti-CD3 | 40.9 | 98.9 | 99.5 | 0.98 | 2.34 | 99.0 |
| EXO vs TRP-CTRL | ||||||
| K562 | 7.8 | 91.7 | 95.7 | 0.88 | 1.32 | 93.2 |
| Anti-CD16 | 15.2 | 98.8 | 94.6 | 0.93 | 1.74 | 96.6 |
| Anti-CD3 | 21.3 | 95.2 | 91.3 | 0.87 | 1.28 | 93.2 |
Optimal cutoff for exocytosis assay (CD107a+ percentage), sensitivity, and specificity obtained through receiver operating characteristic plots comparing 3 different exocytosis assays for patients with variants associated with defective exocytosis (EXO) vs 4 other control groups (IEI, other HYPINFs, 198 HD-CTRLs, and TRP-CTRLs). Youden index, DP, and accuracy were calculated based on the obtained values.HD-CTRL, healthy adult volunteers; TRP-CTRL, healthy adult transport controls.