Figure 4.
Condition of cell migration after treatment with AS101 detected by Transwell assay. 4T1 (A) and CT26 (B) cells (104 cells per well) were placed into a culture insert with a permeable membrane inside a 24-well dish and incubated with 8 μM AS101 for 24 hours. The cells that passed through the permeable membrane were stained with DAPI and measured by fluorescence microscopy (200×). The motility of cells in different groups of 4T1 (C) and CT26 (D) cells was measured. Data represent the mean ± SD (n=3). *** P < 0.001; ** P< 0.01.