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. Author manuscript; available in PMC: 2024 Sep 5.
Published in final edited form as: Cytotherapy. 2020 Dec 7;23(2):119–130. doi: 10.1016/j.jcyt.2020.11.002

Fig. 2. Expression of GXMR-CAR on the surface of human T cells.

Fig. 2.

(A) Flow cytometry plots show anti-CD3 antibody-positive T cells on the y-axis and GXMR-CAR-GFP–expressing T cells on the x-axis. The cells double-positive for the antibody and GFP (82.4%) are GXMR- CAR+ GFP-expressing T cells. (B) Flow cytometry plots show T cells positive for an anti- human Fc antibody on the y-axis and GXMR-CAR-GFP–expressing cells on the x-axis. The cells double-positive for the antibody and CAR-GFP (77.8%) were considered GXMR-CAR+ T cells. Mock-transduced negative control T cells (NoDNA) did not receive the GXMR-CAR construct. Phenotypic analysis of GXMR-CAR+ T cells (C, D): This analysis of GXMR- CAR+ T cells stimulated by using an ImmunoCult human CD3/CD28 T-cell activator was performed after 30 days of in vitro expansion of the cells. (C) PBMCs and (D) GXMR-CAR+ T cells were stained with a mixture of anti-CD3-PE, anti-CD8-PerCP, anti-CCR7-APC, and anti-CD45RA-PE-Vio770 antibodies, and CAR+ T cells were evaluated according to GFP expression. CD3+CD8+ T cells were gated to evaluate the memory cell subsets using anti-CCR7 memory T cells; TEM, effector memory T cells; TEFF, effector T cells; TSCM, stem cell memory