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. Author manuscript; available in PMC: 2024 Sep 5.
Published in final edited form as: Cytotherapy. 2020 Dec 7;23(2):119–130. doi: 10.1016/j.jcyt.2020.11.002

Fig 4. Activation of GXMR-CAR+ T cells after recognition of GXM from Cryptococcus spp..

Fig 4.

(A) GXMR-CAR+ and NoDNA T cells were incubated with a preparation of polysaccharide antigens from the capsule of Cryptococcus spp. at a 1:100 dilution. After 24 h, the images were acquired by bright-field microscopy at 400x magnification. Positive control T cells were activated by using an ImmunoCult human CD3/CD28 T-cell activator (STEMCELL Technologies) according to the manufacturer’s instructions. T cells incubated with medium alone were used as negative control cells. (B and C) GXMR-CAR+ T cells were incubated with soluble GXM from C. neoformans at a dilution of 1:100 and Golgi-stop was added as recommended by manufacturer’s instructions. After 3 h the GXMR-CAR+ T cells were used for detection of granzyme (B) and IFN-γ (C) by flow cytometry. An ImmunoCult human CD3/CD28 T-cell activator (anti-CD3/CD28 stimulus) was used as positive control and medium alone were considered as negative controls.