Figure 3.

Acute mouse model of Chagas disease (T. cruzi Br-Luc) after combination therapy with benznidazole and pyronaridine. (A) In vivo antiparasitic activity (day 7 postinfection) following oral dosing of pyronaridine (PYR) and or benznidazole (Bz). (B) Luminescent signal in mice following the treatment with different drugs or vehicle. Statistical significance was determined using an ordinary one-way ANOVA with Dunnett’s multiple comparisons test against the vehicle as calculated in Graphpad Prism 10.0. Only comparisons that are statistically significant (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001) are shown. Percent inhibition is calculated from the mean of each group as compared to the vehicle and only significant inhibition is shown.