Table 1. Pyronaridine Antiparasitic Activity (IC₅₀) in Different T. cruzi Strains Tested against Intracellular Parasites Infecting C2C12 Host Cells Assessed by Phenotypic Readout^a.

Trypanosoma cruzi strain	DTU	In vitro IC50 (μM)
CA-I/72 (reference strain)	TcI	0.16 ± 0.06
CL-luc	TcVI	>10
Columbiana	TcI	∼0.21
Brazil-luc	TcI	0.195b
Sylvio X10/4	TcI	0.13 ± 0.54

^aSee in vitro infection in the “Methods” section (n ≥ 2, ±SD).

^bAs described previously.⁴⁷