Figure 1.

In vitro characterization of duloxetine following the in silico analyses against human cholinesterases. (A) Single 100 μM concentration screening of duloxetine against human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). (B) Half-maximal inhibitory concentration (IC₅₀) analyses for duloxetine toward human AChE and BChE. The analyses were done at 0.5 and 5 mM concentrations of acetylthiocholine and butyrylthiocholine as preferred substrates of AChE and BChE, respectively. All data are shown as the percent of the enzymes’ activity in the presence of the solvent (vehicle control). Data are shown as mean ± standard deviation (SD). The IC₅₀ analyses were done with GraphPad Prism 9.