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. 2024 Sep 5;19:64. doi: 10.1186/s13024-024-00752-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 11 — Single-cell radiotracing disentangles microglia as biological driver of TSPO-PET desynchronization in an AD mouse. A scRadiotracing workflow. B Percentage of isolated microglia and astrocytes fraction in the single cell suspension of WT (n=5) and App^NL-G-F mice (n=7). C Cellular TSPO tracer uptake in microglia and astrocytes as identified by scRadiotracing (WT: n=8, App^NL-G-F: n=8). Unpaired t-test: **: p < 0.01, ***: p < 0.001. Each point corresponds to a single mouse. D Graphical summary of (B) and (C): the number of cells in 3D brain images is proportional to the isolated cell fraction in the corresponding region; the size of the cells is related to the cellular TSPO tracer uptake in the region (square root relationship for display purpose). The scatter plots display the relationship between the cellular tracer uptake in the forebrain and the hindbrain; each point stands for a single animal; the straight lines represent linear fits calculated per cohort. Line color and thickness are proportional to Fisher’s Z and absolute Fisher’s Z, respectively, both in scatter plots and in 3D representations. Lower absolute Fisher’s Z corresponds to a higher degree of forebrain-hindbrain desynchronization. Estimated contributions of non-microglia/non-astrocyte cell types and gating strategies of quality control are presented in Supplementary Fig. S7