Table 1. Macromolecule-production information.
Source organism | Brucella ovis (strain ATCC 25840, 63/290, NCTC 10512) |
Forward primer | 5′-CTCACCACCACCACCACCATATGGCCGAACCGCTGAAGATCG-3′ |
Reverse primer | 5′-ATCCTATCTTACTCACTTAGCCCGGACGCTTCATGGAGC-3′ |
Cloning vector | BG1861 |
Expression vector | BG1861 |
Expression host | BL21(DE3)R3 Rosetta |
Complete amino-acid sequence of the construct produced | MAHHHHHHAEPLKIALVETLSGPQASTGLLYRAAVLYQLGKINEAGGFNGEKIQILEYDNQGGPVGAADRVKAAIADGAQIIVQGSSSAVAGQITEDVRKYNLRNKGKEVLYLNLGAEALELTGSKCHFYHFRFSPNAAIHFKTVAQGMKDKGILGERAYSINQNYSWGVDVENTVVANAKEIGYEVVDKTLHEVNKIQDFSPYVAKIQAANVDTVFTGNWSNDLLLLMKAASGAGLKAKFATSFLDQPGNIGNAGAIAEGHIVSTPFNPEANGEASMAFAEDYKKVTGHYPSYVEPAAVFGLQLFGEALKNVKPGEGKINTTDIALAIENASVKTPMGDYSMRSDDHQAKFPMVVQEVSKKARIKADGTEYGFLPFKTFTGDESIDPVQESCSMKRPG |