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. 2024 Aug 23;80(Pt 9):193–199. doi: 10.1107/S2053230X24007027

Table 1. Macromolecule-production information.

Source organism Brucella ovis (strain ATCC 25840, 63/290, NCTC 10512)
Forward primer 5′-CTCACCACCACCACCACCATATGGCCGAACCGCTGAAGATCG-3′
Reverse primer 5′-ATCCTATCTTACTCACTTAGCCCGGACGCTTCATGGAGC-3′
Cloning vector BG1861
Expression vector BG1861
Expression host BL21(DE3)R3 Rosetta
Complete amino-acid sequence of the construct produced MAHHHHHHAEPLKIALVETLSGPQASTGLLYRAAVLYQLGKINEAGGFNGEKIQILEYDNQGGPVGAADRVKAAIADGAQIIVQGSSSAVAGQITEDVRKYNLRNKGKEVLYLNLGAEALELTGSKCHFYHFRFSPNAAIHFKTVAQGMKDKGILGERAYSINQNYSWGVDVENTVVANAKEIGYEVVDKTLHEVNKIQDFSPYVAKIQAANVDTVFTGNWSNDLLLLMKAASGAGLKAKFATSFLDQPGNIGNAGAIAEGHIVSTPFNPEANGEASMAFAEDYKKVTGHYPSYVEPAAVFGLQLFGEALKNVKPGEGKINTTDIALAIENASVKTPMGDYSMRSDDHQAKFPMVVQEVSKKARIKADGTEYGFLPFKTFTGDESIDPVQESCSMKRPG