Figure 7.

MITE-containing indel conservation. Five lines were selected among each subpopulation defined by the allele at the local eQTL to look for the conservation of the MITE-containing indel. A) Selected lines are highlighted on a graph representing PIP2;5 expression on the MZ according to the allele at the lead SNP of the local eQTL. B) PCR amplifications using as template gDNA from the chosen hybrids (inbred line × UH007). Middle panel, amplification of the PIP2;5 promoter region (primers shown by blue arrows—top panel) allowing the identification of 2 main alleles associated with the B73 and B104 (lower band) or PH207 (upper band) reference genomes, respectively. Lower panel, amplification figuring the presence of the MITE-containing indel (circled in black) thanks to both an internal and an external primer (shown by orange arrows).