Extended Data Table 1.

Summary of experimental results for designed proteins

The second column shows the number of designs experimentally tested for the fold in the leftmost column. The subsequent columns give the number of designs that satisfy each experimental characterization, which was performed sequentially from the left to the right. The successful designs are defined as those that satisfy all criteria and are expected to fold into correct fold. The details of the results are shown in Supplementary Tables 2-6.

^†Expression and solubility were assessed by SDS-PAGE and mass spectrometry.

^‡SEC-MALS was used to determine oligomerization state. The number of designs in which the main peak of the absorbance at 280 nm corresponds to the monomeric state was counted.

^*1H-¹⁵N HSQC spectra were collected.