Table 1.

Experimental design and the responses obtained using five factorial central composite model.

Standard	Factors				Responses
	IR (%)	Light intensity (µmol/m2/s)	CO2 (%)	Harvest time (days)	Algal biomass (cells/mL)	Lipid productivity (total FL/mL)
1	43.75	112.5	2	4.5	2.4 × 105	5.5 × 1010
2	81.25	112.5	2	4.5	3.2 × 106	1.8 × 1011
3	43.75	237.5	2	4.5	3.9 × 106	1.7 × 1011
4	81.25	237.5	2	4.5	5.2 × 106	1.2 × 1011
5	43.75	112.5	4	4.5	8.5 × 105	1.5 × 1010
6	81.25	112.5	4	4.5	4.2 × 106	3.1 × 1010
7	43.75	237.5	4	4.5	4.5 × 106	1.1 × 1012
8	81.25	237.5	4	4.5	9.2 × 106	8.6 × 1010
9	43.75	112.5	2	11.5	1.1 × 106	1.6 × 1012
10	81.25	112.5	2	11.5	4.5 × 106	3.3 × 1012
11	43.75	237.5	2	11.5	4.1 × 106	3.1 × 1012
12	81.25	237.5	2	11.5	1.1 × 107	3.6 × 1012
13	43.75	112.5	4	11.5	4.4 × 106	1.8 × 1011
14	81.25	112.5	4	11.5	8.3 × 106	1.2 × 1011
15	43.75	237.5	4	11.5	3.4 × 106	3.2 × 1010
16	81.25	237.5	4	11.5	1.9 × 107	2.5 × 1012
17	25	175	3	8	4.3 × 106	8.9 × 1011
18	100	175	3	8	8.5 × 106	3.5 × 1012
19	62.5	50	3	8	4.1 × 106	3.2 × 1010
20	62.5	300	3	8	1.5 × 106	5.1 × 1010
21	62.5	175	1	8	3.4 × 106	1.1 × 1011
22	62.5	175	5	8	4.2 × 106	1.2 × 1011
23	62.5	175	3	1	2.1 × 105	3.3 × 1010
24	62.5	175	3	15	2.0 × 106	2.8 × 102
25	62.5	175	3	8	8.3 × 106	1.5 × 1012
26	62.5	175	3	8	8.0 × 106	1.6 × 1012
27	62.5	175	3	8	8.2 × 106	1.6 × 1012
28	62.5	175	3	8	8.0 × 106	1.5 × 1012
29	62.5	175	3	8	8.2 × 106	1.5 × 1012
30	62.5	175	3	8	8.5 × 106	1.6 × 1012

A—Inoculation ratio of bacteria to algae (IR) (25–100%).

B—Light intensity (50–300 µmol/m²/s).

C—Carbon dioxide concentration (1–5%, v/v).

D—Harvest time (1–15 days).