Fig. 5. TMEM106B regulates GALC activity in the brain.

a Enzymatic activity of forebrain, brainstem, and cerebellar extracts of WT and Tmem106b^−/− mice at 13 months of age toward MUGAL substrate in the absent or presence of 12.5 μM AgNO₃. n = 6 mice (2 males and 4 females) per genotype. *p = 0.0137 (forebrain, 0 μM), **p = 0.0016 (forebrain, 12.5 μM), **p = 0.0035 (brainstem, 0 μM), *p = 0.0383 (brainstem, 12.5 μM); two-tailed unpaired t-test. b GALC activity of forebrain extracts of WT and Tmem106b^−/− mice at 13 months of age measured using HMGal. n = 6 mice (2 males and 4 females) per genotype. **p = 0.0017; two-tailed unpaired t-test. c Correlation between the results of GALC activity assay using MUGAL and HMGal. d GALC activity of digitonin-soluble brainstem lysates of WT and Tmem106b^−/− mice at 13 months of age measured with HMGal. n = 6 mice (2 males and 4 females) per genotype. **p = 0.0022; two-tailed unpaired t-test. e Representative blots of the digitonin-soluble brainstem lysates using anti-GALC and anti-GAPDH antibodies. The asterisks indicate non-specific bands. Whole brain lysates from ~40-day-old wild type and twitcher (GALC-deficient) mice were used as positive and negative controls (PC and NC). f Quantification of the blots in (e). Mean ± SEM, n = 6 mice (2 males and 4 females) per genotype. Two genotypes were not significantly different by two-tailed unpaired t-test.