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. 2024 Sep 5;14:20664. doi: 10.1038/s41598-024-71248-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — Stability and degradation of RecA splicing products. (A, B) Mid-log Mtb cultures were treated with Mitomycin C (MMC) for 24 h, then treated with chloramphenicol (CAM) for the indicated times to block new protein synthesis. (A) Western blot analysis of intein levels over time. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP. (B) Western blot analysis of ligated extein levels over time. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP. (C, D) Mtb cultures were treated ( +) with bortezomib (BTZ) to block proteasome activity or left untreated (−). (C) Western blot analysis of intein levels over time. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP. (D) Western blot analysis of ligated extein, LE, NEA, and NE levels after BTZ treatment. Top: Representative blot. Bottom: Quantification of 3 biological repeats normalized to GFP; ** indicates p < 0.01.