Fig. 6. Impacts of CuPAHMQ on the MCF-7 cell cycle and apoptosis using flow cytometry analysis. (A and B) Cell cycle phases for (A) untreated MCF-7 cells, (B) treated MCF-7 cells, and (C) the DNA content. (D–F) Impacts of a 24 hour treatment with an IC50 dose of the CuPAHMQ complex on the distribution of MCF-7 cells across four distinct quadrants, early apoptosis (Q4; Ann V+/PI), viable cells (Q3; Ann V, PI), late apoptosis (Q2; Ann V+, PI+), and necrosis (Q1; Ann V, PI+) using annexin V-FITC assay.
