Fig. 7.

Effect of HSF1 inhibition on the growth of intrahepatic cholangiocarcinoma cell lines. A Inhibition of HSF1 via specific small-interfering RNA in KKU-M156, HuCCT1, and KKU-M213 human intrahepatic cholangiocarcinoma (iCCA) cell lines, as assessed by Western blot analysis. B-D BrdU incorporation assay was conducted on B KKU-M156, C HuCCT1, and D KKUM-213 cells. Analysis was conducted 48 h after siRNA administration. Two siRNA concentrations (25 and 50 nm) were applied. Scramble-treated cells served as control. E–G KKU-M156, HuCCT1, and KKUM-213 cells were also treated for 48 h with 10 µM KRIBB-11, an HSF1 inhibitor, and BrdU incorporation was assessed. DMSO-treated cells served as controls. The values in optical densities (OD) at 450 nm are presented. All results are expressed as mean ± SD of three independent experiments in triplicate. For statistical analysis, Tukey’s multiple comparisons test was performed (*** p < 0.0001, vs. scramble and DMSO). Abbreviation: si-HSF1, small-interfering RNA against HSF1