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. 2024 Sep 5;29:118. doi: 10.1186/s11658-024-00627-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — In vitro experiments reveal that hsa_circ_0000520 inhibits bladder cancer cell invasion, migration, and VM formation. A qRT-PCR analysis of the transfection efficiency of overexpressed hsa_circ_0000520 lentivirus in T24 cells. B qRT-PCR analysis of the transfection efficiency of knocked down hsa_circ_0000520 lentivirus in UMUC3 cells. C Scratch assay analyzing the impact of hsa_circ_0000520 overexpression on the migration ability of T24 cells. D Transwell assay analyzing the effect of hsa_circ_0000520 overexpression on the migration ability of T24 cells. E Transwell assay analyzing the effect of hsa_circ_0000520 overexpression on the invasion ability of T24 cells. F VM formation assay analyzing the impact of hsa_circ_0000520 overexpression on the VM formation ability of T24 cells. G Scratch assay analyzing the effect of knocked down hsa_circ_0000520 on the migration ability of UMUC3 cells. H Transwell assay analyzing the effect of knocked down hsa_circ_0000520 on the migration ability of UMUC3 cells. I Transwell assay analyzing the effect of knocked down hsa_circ_0000520 on the invasion ability of UMUC3 cells. J VM formation assay analyzing the effect of knocked down hsa_circ_0000520 on the VM formation ability of UMUC3 cells. β-actin serves as an internal reference. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001