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. 2024 Sep 5;29:118. doi: 10.1186/s11658-024-00627-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Hsa_circ_0000520 binds to Lin28a and reduces its protein stability. A RNA pulldown product validation by WB confirms the pulldown of Lin28a by hsa_circ_0000520. B RIP reverse analysis demonstrates the binding between Lin28a and hsa_circ_0000520. C Fluorescence colocalization analysis reveals the co-localization of hsa_circ_0000520 with Lin28a. D, E qRT-PCR analysis of the impact of overexpression and knockdown of hsa_circ_0000520 on Lin28a mRNA levels. F, G WB analysis of the effect of overexpression and knockdown of hsa_circ_0000520 on Lin28a protein levels. H WB analysis of changes in Lin28a protein levels after streptavidin treatment in the overexpression group of hsa_circ_0000520. I WB analysis of changes in Lin28a protein levels after streptavidin treatment in the knockdown group of hsa_circ_0000520. J WB analysis of changes in Lin28a protein levels after MG132 treatment in the overexpression group of hsa_circ_0000520. K WB analysis of changes in Lin28a protein levels after MG132 treatment in the knockdown group of hsa_circ_0000520. L Co-IP analysis of changes in Lin28a ubiquitination levels after MG132 treatment in the overexpression group of hsa_circ_0000520. M Co-IP analysis of changes in Lin28a ubiquitination levels after MG132 treatment in the knockdown group of hsa_circ_0000520. β-actin serves as an internal reference. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001