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. 2024 Sep 5;29:118. doi: 10.1186/s11658-024-00627-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Hsa_circ_0000520/Lin28a regulates the PI3K/AKT pathway by enhancing PTEN mRNA stability. A qRT-PCR analysis validating the transfection efficiency of Lin28a overexpression and knockdown. B WB analysis confirming the transfection efficiency of Lin28a overexpression and knockdown. C qRT-PCR analysis showing that Lin28a overexpression partially reverses the impact of hsa_circ_0000520 overexpression on PTEN mRNA expression levels. D qRT-PCR analysis demonstrating that Lin28a knockdown partially reverses the impact of hsa_circ_0000520 knockdown on PTEN mRNA expression levels. E WB analysis revealing that Lin28a overexpression partially reverses the impact of hsa_circ_0000520 overexpression on PTEN, PI3K, AKT, mTOR, p-PI3K, p-AKT, and p-mTOR protein levels. F WB analysis indicating that Lin28a knockdown partially reverses the impact of hsa_circ_0000520 knockdown on PTEN, PI3K, AKT, mTOR, p-PI3K, p-AKT, and p-mTOR protein levels. β-actin serves as an internal reference. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001