Fig. 2. Nociceptors suppress leukocyte influx during CRKP lung infection.

(A and B) H&E-stained sections of lungs: ×0.9 to ×1.1 magnification (A) and ×20 magnification of selected area (in rectangles) (B) of control and Trpv1^DTA mice with PBS inoculation and after CRKP infection (24 hpi). Representative images were selected from at least 12 lobes of n = 3 to 4 mice in each group. Scale bars are shown in each figure. (C to E) Total live cells (C), representative flow cytometry plots of total leukocytes (CD45⁺ cells) (D), and total CD45⁺ cells (E), in BALF of Trpv1^DTA and control mice at 6, 12, and 24 hpi with CRKP. Data in (C) and (E) are the means ± SEM and involve n = 4 mice per group for 0-, 6-, and 12-hour time course analyses and n = 7 to 8 mice per group for 24-hour time course analyses. Statistical analysis was done by two-way ANOVA of two-stage liner step-up procedure of Benjamini, Krieger, and Yekutieli posttests with the following significance levels: **P < 0.01. BAL, bronchoalveolar lavage.