| Gene |
Primer |
Amplicon |
| Primers for species identification |
| nuc- Staphylococcus haemolyticus |
F- TAGTGGTAGGCGTATTAGCC |
434 bp |
| R- ACGATATTTGCCATTCGGTG |
| nuc- Staphylococcus epidermidis |
F- TTGTAAACCATTCTGGACCG |
251 bp |
| R- ATGCGTGAGATACTTCTTCG |
| Primers for methicillin resistance |
| mecA |
F- GTAGAAATGACTGAACGTCCGATAA |
310 bp |
| R- CCAATTCCACATTGTTTCGGTCTAA |
| mecC |
F- GAAAAAAAGGCTTAGAACGCCTC |
138 bp |
| R- GAAGATCTTTTCCGTTTTCAGC |
| Primers for virulence factors of coagulase-negative Staphylococci
|
| aap |
F- AAACGGTGGTATCTTACGTGAA |
466 bp |
| R- CAATGTTGCACCATCTAAATCAGCT |
|
| fbe |
F- CTACAAGTTCAGGTCAAGGACAAGG |
273 bp |
| R- GCGTCGGCGTATATCCTTCMG |
|
| atlE |
F- CAACTGCTCAACCGAGAACA |
682 bp |
| R- TTTGTAGATGTTGTGCCCCA |
|
| embp |
F- AGCGGTACAAATGTCAAT |
455 bp |
| R- AGAAGTGCTCTAGCATCATCC |
|
| icaAB |
F- TTATCAATGCCGCAGTTGTC |
516 bp |
| R- GTTTAACGCGAGTGCGCTAT |
|
| Primers for Staphylococcal cassette chromosome mec (SCCmec) typing |
| β |
F- ATTGCCTTGATAATAGCCYTCT |
937 bp |
| a3 |
R- TAAAGGCATCAATGCACAAACACT |
|
| ccrC |
F- CGTCTATTACAAGATGTTAAGGATAAT |
518 bp |
| ccrC |
R- CCTTTATAGACTGGATTATTCAAAATAT |
|
| 1272 |
F- GCCACTCATAACATATGGAA |
415 bp |
| 1272 |
R- CATCCGAGTGAAACCCAAA |
|
| 5RmecA |
F- TATACCAAACCCGACAACTAC |
359 bp |
| 5R431 |
R- CGGCTACAGTGATAACATCC |
|
