Table 1. The PC properties of 15 proteins using the ProtParam free web server.
mNeph: mouse nephrin; hNeph: human nephrin; hMMP 2: human matrix metalloproteinase 2; hMMP 9: human matrix metalloproteinase 9; hRBP 3: human retinol-binding protein 3; hRBP 4: human retinol-binding protein 4; hKLK 1: human kallikrein 1; hUromodulin: human uromodulin; hIGFBP 7: human insulin-like growth factor binding protein 7; hCystatin C: human cystatin C; hPodocin: human podocin; hBeta arrestin 1: human beta arrestin 1; hVANGL2: human vang-like protein 2; hDynamin 1: human dynamin 1; hTENC1: human tensin-like C1 domain-containing phosphatase; MW■: molecular weight of protein; P1◊: theoretical isoelectric point of protein; NR◘: total number of negatively charged residues present in the protein; PRׁ: total number of positively charged residues present in the protein; Ext.co1▼: extinction coefficient assuming all pairs of Cys residues form cystines; Ext.co2▲: extinction coefficient assuming all Cys residues are reduced; GRAVY*: grand average of hydropathicity of the protein; PC: physicochemical
| Proteins | MW■ | P1◊ | NR◘ | PRׁ | Ext.co1▼ | Ext.co2▲ | Instability index | Aliphatic index | GRAVY* |
| mNeph | 1180.4 | 6.75 | 1 | 1 | - | - | 58.29 | 146.00 | 0.520 |
| hNeph | 134742.2 | 5.42 | 139 | 106 | 166490 | 165240 | 46.04 | 86.74 | -0.246 |
| hMMP 2 | 17936.9 | 4.85 | 25 | 14 | 32430 | - | 15.46 | 69.75 | -0.461 |
| hMMP 9 | 17911.9 | 5.12 | 22 | 10 | 33920 | - | 27.70 | 77.50 | -0.281 |
| hRBP 3 | 15781.0 | 5.97 | 20 | 17 | 24075 | 23950 | 30.16 | 86.42 | -0.484 |
| hRBP 4 | 21225.8 | 5.27 | 28 | 24 | 34295 | 33920 | 33.50 | 65.35 | -0.592 |
| hKLK 1 | 26405.6 | 4.62 | 34 | 13 | 46575 | 45950 | 39.66 | 79.79 | -0.229 |
| hUromodulin | 76456.7 | 5.60 | 82 | 67 | 96230 | 95230 | 24.75 | 78.94 | -0.258 |
| hIGFBP7 | 50266.4 | 7.62 | 47 | 49 | 46390 | 43890 | 41.44 | 53.58 | -0.443 |
| hCystatin C | 13333.1 | 8.75 | 12 | 15 | 11710 | 11460 | 45.63 | 64.17 | -0.507 |
| hPodocin | 42200.9 | 8.95 | 45 | 51 | 24325 | 23950 | 53.38 | 93.47 | -0.170 |
| hBeta arrestin-1 | 47051.6 | 5.84 | 66 | 59 | 19495 | 19370 | 36.26 | 86.67 | -0.515 |
| hVANGL2 | 59714.5 | 9.27 | 61 | 72 | 67060 | 66810 | 48.41 | 90.36 | -0.297 |
| hDynamin 1 | 39333.0 | 5.89 | 48 | 43 | 10555 | 10430 | 40.62 | 97.45 | -0.310 |
| hTENC1 | 17191.5 | 9.18 | 12 | 16 | 14105 | 13980 | 46.52 | 72.10 | -0.315 |