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. 2024 Sep 6;15(9):655. doi: 10.1038/s41419-024-07013-w

Fig. 1. RAB17 regulates EC cell proliferation.

Fig. 1

A CCK-8 assays of Ishikawa and HEC-1A cell lines transfected with normal control siRNA (NC-si) or RAB17 siRNA (RAB17-si). B CCK-8 assays of Ishikawa and HEC-1A cells infected with normal control lentivirus (Lv-NC) or RAB17 overexpression lentivirus (Lv-RAB17). C Results from the EdU assays of Ishikawa and HEC-1A cells transfected with NC-si or RAB17-si for 48 h. Scale bars, 200 μm. D Results from the EdU assays of Ishikawa and HEC-1A cells infected with Lv-NC or Lv-RAB17 for 48 h. Scale bars, 200 μm. E Western blot analysis and (F) qRT–PCR analysis of RAB17 expression in Ishikawa and HEC-1A cells cultured in hyperglycemic (Hyper) or hypoglycemic (Hypo) medium for 72 h, respectively. GAPDH was used as an internal control. All the above assays were independently performed in triplicate (N = 3). The data are presented as the means ± SDs. The statistical analyses were performed by two-tailed unpaired Student’s t tests. **P < 0.01, and ***P < 0.001.