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. 2024 Sep 6;15(9):655. doi: 10.1038/s41419-024-07013-w

Fig. 2. RAB17 regulates ferroptosis in EC cells.

Fig. 2

A Single-gene GSEA of RAB17 based on TCGA dataset. Representative images of immunofluorescence staining with (B) an ROS probe and (C) a C11-BODIPY probe in Ishikawa cell lines transfected with NC-si or RAB17-si. Scale bars, 200 μm. D GSH, SOD, and MDA levels in Ishikawa cell lines transfected with NC-si or RAB17-si. E GSH, SOD, and MDA levels in Ishikawa cell lines infected with Lv-NC or Lv-RAB17. F Representative images of immunofluorescence staining with a JC-1 probe in Ishikawa cell lines transfected with NC-si or RAB17-si. Scale bars, 200 μm. G Representative transmission electron microscopy images of Ishikawa and HEC-1A cells transfected with NC-si or RAB17-si. H Representative transmission electron microscopy images of Ishikawa and HEC-1A cells infected with Lv-NC or Lv-RAB17. I Western blot analysis of designated marker proteins for ferroptosis in Ishikawa cells transfected with NC-si or RAB17-si. J Western blot analysis of designated ferroptosis marker proteins in Ishikawa cells infected with Lv-NC or Lv-RAB17. GAPDH was used as an internal control. All the above assays were independently performed in triplicate (N = 3). The data are presented as the means ± SDs. The statistical analyses were performed by two-tailed unpaired Student’s t tests. *P < 0.05, **P < 0.01, and ***P < 0.001.