Skip to main content
. 2024 Sep 6;15(9):655. doi: 10.1038/s41419-024-07013-w

Fig. 3. RAB17 regulates TFRC expression in EC cells at the posttranscriptional level.

Fig. 3

A RAB17-binding proteins predicted based on the BioGRID, IntAct, MINT, ELM, and STRING databases. B Western blot analysis of TFRC expression in Ishikawa and HEC-1A cells transfected with NC-si or RAB17-si. C Western blot analysis of TFRC expression in Ishikawa and HEC-1A cells infected with Lv-NC or Lv-RAB17. D Representative images of IF staining showing TFRC expression in Ishikawa and HEC-1A cells transfected with NC-si or RAB17-si. Scale bars, 200 μm. E qRT–PCR analysis of TFRC expression in Ishikawa and HEC-1A cells transfected with NC-si or RAB17-si. F qRT–PCR analysis of TFRC expression in Ishikawa and HEC-1A cells infected with Lv-NC or Lv-RAB17. GAPDH was used as an internal control. All the above assays were independently performed in triplicate (N = 3). The data are presented as the means ± SDs. The statistical analyses were performed by two-tailed unpaired Student’s t tests. ***P < 0.001.