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. 2024 Sep 6;14:20840. doi: 10.1038/s41598-024-69721-9

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© The Author(s) 2024, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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The PC plot (A) Dispersion estimates (B) histogram (C) and MA plot (D) were created by DESeq2 tool of E-MTAB-4993 dataset. (A) PC plot shows two phenotypes: ER+ and TN. They are grouped on the basis of expression. (B) Dispersion estimates quantify the level of variability in gene expression across samples. Blue dots represent low dispersion estimates of genes and it shows the gene expression is relatively stable while the black dots represent high dispersion estimates. The red line shows the mean or median dispersion estimates. The blue dot close to red line indicated stable expression and the black dots close to red line suggests that the expression values are more variable.(C) Histogram shows the DEG’s grouped into bins or the frequency of genes. (D)MA plot the differences between measurements based on ER+ and TN by transforming the data by using log ratio and mean average. The red color shows the dispersion of differentially expressed genes while grey color shows no variation.