Fig. 1.

Key principles and developments toward state-of-the-art DIA-MS.A, most common isolation window schemes in DIA-MS. B, implementation of DIA-MS over time on different instrument platforms and their usage approximated by citations in Google Scholar. C, schematic overview of state-of-the-art MS instruments with their respective key developments and features highlighted in bold text. The ion flux through the respective ion optics is depicted as a red line with the direction from left to right. D, state-of-the-art DIA-MS strategies based on respective key features of each instrument type. DIA, data-independent acquisition; IM, ion mobility; MS, mass spectrometry.