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. 2024 Aug 23;2024:10.17912/micropub.biology.001268. doi: 10.17912/micropub.biology.001268

Source & catalog number

Dilution

Animal and clonality

Immunogen

Species reactivity

Tested WB applications by the company

Millipore, ST1202

1:1000, TBS-0.1% Tween, 2% milk

Mouse Monoclonal

A recombinant protein corresponding to amino acids 1-120 of mouse PGC-1α

Mouse, human and rat

Tested in mouse, human and rat

Invitrogen, PA5-72948

1:1000 in TBS- 0.1% Tween, 5% BSA

Rabbit Polyclonal

A synthetic peptide corresponding to amino acids 400-550 of human PGC-1α protein (#Q9UBK2)

Hamster, human, and rat, but published in human, mouse, and rat

Tested in human and mouse cell lines: A-431, HeLa, HepG2, NIH/3T3, as well as in human adipose and skeletal muscle

Abcam, ab54481

1:1000 in TBS- 0.1% Tween, 5% BSA

Rabbit Polyclonal

A synthetic peptide corresponding to amino acids 750-798 of human PGC-1α (#Q9UBK2)

Human and mouse

Tested in human and mouse

ABClonal, A19674

1:1000 in TBS- 0.1% Tween, 5% BSA

Rabbit Monoclonal

A synthetic peptide corresponding to amino acids 699-798 of human PGC-1α/β (NP_037393.1)

Mouse and rat

Tested in mouse liver and rat heart

ABClonal, A12348

1:1000 in TBS- 0.1% Tween, 5% BSA

Rabbit Polyclonal

A recombinant fusion protein corresponding to amino acids 610-798 of human PGC-1α (NP_037393.1, #Q9UBK2)

Human, mouse and rat

Tested in 293T cells, mouse skeletal muscle, mouse kidney and rat kidney

ABClonal, A20995

1:1000 in TBS- 0.1% Tween, 5% BSA

Rabbit Monoclonal

A recombinant fusion protein corresponding to amino acids 340-480 of human PGC-1α (NP_037393.1, #Q9UBK2)

Human, mouse and rat

Tested in HeLa cells, mouse kidney, rat kidney and rat stomach

ABClonal, A11971

1:1000 in TBS- 0.1% Tween, 5% BSA

Rabbit Polyclonal

A recombinant fusion protein corresponding to amino acids 610-710 of human PGC-1α (NP_037393.1, #Q9UBK2)

Human, mouse and rat

Tested in mouse kidney, mouse heart and rat heart