Fig. 4.

Immunofluorescence with the proliferative marker, Ki67. Human OVCAR4 cells were incubated for 48 h. with the IC50s of P from different geographical origin either alone or co-supplemented with vitamin D using the results obtained from Drug Reduction Indices analysis which were briefly as follow: 38.7045 μg/mL for Egy P + 0.01480 μg/mL for Vit D, 47.4519 μg/mL for Ger P + 0.01815 μg/mL for Vit D, and 36.9895 μg/mL for Fre P + 0.01415 μg/mL for Vit D. The left column was stained with DAPI to indicate the nuclei; the middle column was stained with Ki67 to indicate proliferation; and the right column contains the merged images. Scale bar: 50 μm