Figure 1.

Localization and expression of A_2AR in full-thickness excisional wounds of WT mice. (A) Experimental procedure. (B) A_2AR levels were detected by western blotting at 0, 6, 9 and 15 days postwounding. **p < 0.01, *p <0.05 (n=3); NS, not significant. Immunohistochemistry for A_2AR (red, white arrow) in the wound and surrounding area at 9 days postwounding (C) and its quantitative analysis (D) in WT mice. **p < 0.01 (n=5); NS, not significant. The lower panel shows higher magnification sections of the white squares in the upper panel. Scale bar, 50 µm. Immunohistochemistry for CD31 (E), vimentin (F), CD11b (G), F4/80 (H) (green) and A_2AR (red) in wound granulation tissue at 9 days postwounding in WT mice. The right panel shows higher magnification sections of the white squares on the left, and the white arrow indicates that the green fluorescent-labeled cells colocalized with the red fluorescent-labeled cells. Short scale bar, 50 µm; long scale bar, 50 µm.