Figure 4.

The expression of c-Ski in A_2AR KO and EC-A_2AR KO mice after full-thickness wounding. (A) Experimental procedure. (B) Immunohistochemistry for c-Ski (green, white arrow) in the granulation tissue of the wound and subcutaneous tissue of the surrounding wound at 9 days postwounding. The lower panel shows higher magnification sections of the black squares in the upper panel. Scale bar, 50 µm. (C) Double-label immunofluorescence for c-Ski (red) and CD31 (green) in the granulation tissue of the wound at 9 days postwounding revealed the colocalization of c-Ski and CD31 (white arrow). The surrounding area is a higher magnification section of the white square. Short scale bar, 50 µm; long scale bar, 50 µm. Expression of c-Ski detected by (D) real-time PCR (n = 9) and (E) western blot analysis (n = 3) at 0, 6, 9 and 15 days postwounding. **p < 0.01; NS, not significant. (F) Double-label immunofluorescence for c-Ski (red) and CD31 (green) in the granulation tissue of the wound at 9 days postwounding in each group. The white arrow indicates the colocalization of c-Ski and CD31. Scale bar, 50 µm. (G) Quantitative analysis of (F). **p < 0.01 (n=5); NS, not significant.