Figure 5.

Changes in the proangiogenic effect in HMECs by the application of an A_2AR agonist in vitro. (A) Immunohistochemistry of A_2AR (red) in HMECs. Scale bar, 50 µm. (B) Cell proliferation was measured by the CCK-8 assay 24 h after treatment with different concentrations of CGS21680. **p < 0.01 (n=9); NS, not significant. (C) Representative images of cell migration evaluated by scratch wound healing assays at 0, 24, 48 and 72 h after CGS21680 treatment. Scale bar, 50 µm. (D) Representative images of tube formation 24 h after treatment with CGS21680. Scale bar, 50 µm. (E) Quantitative analysis of (D). **p < 0.01 (n=5); NS, not significant.